A visual demonstration of convergence properties of cooperative coevolution

We introduce a model for cooperative coevolutionary algorithms (CCEAs) using partial mixing, which allows us to compute the expected long-run convergence of such algorithms when individuals ’ fitness is based on the maximum payoff of some N evaluations with partners chosen at random from the other population. Using this model, we devise novel visualization mechanisms to attempt to qualitatively explain a difficult-to-conceptualize pathology in CCEAs: the tendency for them to converge to suboptimal Nash equilibria. We further demonstrate visually how increasing the size of N, or biasing the fitness to include an ideal-collaboration factor, both improve the likelihood of optimal convergence, and under which initial population configurations they are not much help

Calcium Uptake and Proton Transport by Acidocalcisomes of Toxoplasma gondii

Acidocalcisomes are acidic calcium stores found in diverse organisms, being conserved from bacteria to humans. They possess an acidic matrix that contains several cations bound to phosphates, which are mainly present in the form of short and long polyphosphate chains. Their matrix is acidified through the action of proton pumps such as a vacuolar proton ATPase and a vacuolar proton pyrophosphatase. Calcium uptake occurs through a Ca2+/H+ countertransporting ATPase located in the membrane of the organelle. Acidocalcisomes have been identified in a variety of microorganisms, including Apicomplexan parasites such as Plasmodium and Eimeria species, and in Toxoplasma gondii. We report the purification and characterization of an acidocalcisome fraction from T. gondii tachyzoites after subcellular fractionation and further discontinuous iodixanol gradient purification. Proton and calcium transport activities in the fraction were characterized by fluorescence microscopy and spectrophotometric methods using acridine orange and arsenazo III, respectively. This work will facilitate the understanding of the function of acidocalcisomes in Apicomplexan parasites, as we can now isolate highly purified fractions that could be used for proteomic analysis to find proteins that may clarify the biogenesis of these organelles